Time-of-Flight Mass Spectrometer (TOF-MS) is a high-end analytical instrument that achieves precise mass-to-charge ratio measurement based on differences in ion flight time. Featuring core advantages such as a wide mass analysis range, fast analysis speed, high resolution, high sensitivity and full-spectrum simultaneous acquisition, it is widely applied in proteomics, metabolomics, food safety testing, environmental monitoring, drug analysis, geological exploration, life science research and other fields, serving as a key device in modern analytical science and clinical detection. The high-voltage pulse power supply is a critical core component of TOF-MS, providing nanosecond-level high-voltage pulse output for ion repulsion, ion gates, reflectors and deflectors in the ion source to realize precise ion extraction, mass gating, trajectory focusing and energy compensation. Its pulse rising/falling edge steepness, amplitude accuracy, timing jitter and multi-channel synchronization accuracy directly determine the mass resolution, mass accuracy, sensitivity and detection limit of the mass spectrometer. TOF-MS imposes extremely stringent nanosecond-level synchronization requirements on high-voltage pulse power supplies. The ion repulsion pulse typically requires continuously adjustable output amplitude of 0~5 kV, continuously adjustable pulse width of 50 ns~10 μs, pulse rising/falling edge ≤ 10 ns, multi-channel synchronization accuracy ≤ 1 ns, timing jitter ≤ 500 ps, and amplitude accuracy better than ±0.5%. Failure to meet these specifications will increase time dispersion and initial energy dispersion during ion extraction, broaden mass spectral peaks, severely reduce mass resolution and accuracy, and even prevent accurate qualitative and quantitative analysis of target compounds. Traditional high-voltage pulse power supplies adopt topologies such as direct high-voltage switch driving and Pulse Forming Networks (PFN), suffering from slow pulse edges, large timing jitter, low multi-channel synchronization accuracy and poor long-term stability, which cannot satisfy the nanosecond-level synchronization demands of high-end TOF-MS. Relevant designs must strictly comply with standards including GB/T 35410-2017 *General Technical Specifications for Time-of-Flight Mass Spectrometers*, GB/T 37837-2019 *Performance Measurement Methods for Mass Spectrometers*, and JJF 1955-2021 *Calibration Specification for Nanosecond Pulse Voltages*, while meeting the core requirements of mass spectrometers for high resolution, high stability and multi-channel synchronization. Targeting the key application needs and technical challenges of high-voltage pulse power supplies for TOF-MS, this methodology establishes a full-process general technical framework covering nanosecond pulse topology design, multi-channel synchronization control, low-jitter driving optimization, precise pulse waveform regulation and long-term stability design. It accommodates the high-voltage pulse power demands of various MALDI-TOF, ESI-TOF and TOF-TOF mass spectrometers, providing standardized design criteria for domestic substitution and performance improvement of high-end Chinese TOF-MS instruments. Addressing the core design challenges of nanosecond fast-edge pulses, sub-nanosecond synchronization and ultra-low timing jitter in TOF-MS applications, this methodology adopts the main architecture of **modular solid-state Marx generator topology + all-fiber synchronous triggering + FPGA hardware-level timing control**, combined with low-parasitic layout and high-speed driving optimization. It fundamentally overcomes the traditional limitations of poor synchronization accuracy, large jitter and slow pulse edges, achieving fast pulse edges within 10 ns, timing jitter below 500 ps and multi-channel synchronization accuracy within 1 ns, which fully meets the full-operation requirements of high-end TOF-MS. The design follows five core criteria: First, a modular all-solid-state Marx generator topology is adopted as the foundation for nanosecond high-voltage fast-edge pulses. This topology charges multi-stage low-voltage energy storage capacitors in parallel and discharges them in series to directly generate high-amplitude high-voltage pulses without high-voltage pulse transformers, completely eliminating pulse edge distortion, tailing and delay caused by transformer leakage inductance and distributed capacitance. It enables precise nanosecond pulse edge control and flexible parameter adjustment tailored for TOF-MS. Each Marx stage features a fully symmetrical circuit with independent energy storage capacitors, high-speed power switches, freewheeling diodes and current-limiting charging components. Uniform electrical parameters and layout across all stages ensure consistent switching characteristics and discharge impedance, preventing asynchronous switching, slow edges and unstable amplitude. High-speed GaN HEMTs are selected as power switches; compared with conventional Si/SiC MOSFETs, GaN devices offer ultra-low gate charge, ultra-fast switching with rise/fall time within 2 ns and zero reverse recovery loss, perfectly supporting nanosecond pulse control. The voltage stress per stage equals total output divided by stage number, allowing flexible configuration for 0~5 kV or 0~10 kV outputs. Adjustable capacitor capacity modifies pulse width and load capability for different ion optical modules. A constant-current charging topology with isolated DC-DC converters provides independent precise charging for each stage, ensuring uniform capacitor voltage under varying pulse repetition frequencies and widths. Closed-loop voltage control enables continuous 0~100% amplitude adjustment with accuracy better than ±0.5%. Second, the all-fiber synchronous triggering and hardware-level timing control architecture ensures sub-nanosecond multi-channel synchronization and ultra-low jitter. A three-tier synchronization scheme of **high-precision OCXO + FPGA hardware timing generation + all-fiber trigger transmission** eliminates delay, jitter and interference from electrical signal paths. A high-stability oven-controlled crystal oscillator above 100 MHz with daily stability ≤ ±10 ppb and phase noise ≤ −160 dBc/Hz@1 kHz provides an ultra-low-jitter clock reference. All pulse generation, triggering and delay control are implemented purely within FPGA hardware logic without software intervention, delivering timing resolution below 100 ps and eliminating software latency. Amplitude, width, delay and repetition frequency are flexibly configurable via host software for different analytical modes. All trigger signals are converted into optical signals, transmitted through fiber links to each Marx driver and reconverted locally; fiber transmission achieves delay jitter ≤ 100 ps and full immunity to electromagnetic interference, ground potential differences and signal reflection, ensuring multi-channel synchronization ≤ 1 ns and timing jitter ≤ 500 ps for accurate ion extraction. Third, nanosecond pulse waveform optimization and low-parasitic design guarantee waveform integrity for fast-edge pulses. Low-parasitic stacked busbar layout minimizes discharge loop parasitic inductance below 1 nH via closely coupled reverse-current busbars, reducing edge attenuation, oscillation and tailing to secure rise/fall edges ≤ 10 ns. Symmetric component placement ensures identical discharge path length and impedance across all stages to maintain synchronous switching. An adjustable impedance matching network at the output matches the spectrometer load with damping resistors and non-inductive capacitors to eliminate reflection and ringing in transmission lines. High-voltage 50 Ω coaxial cables maintain continuous impedance during pulse propagation to avoid waveform distortion. Active clamping and RCD snubbers suppress voltage spikes and overshoot; output active clamping absorbs excess energy dynamically, limiting pulse overshoot ≤ 0.5% and flat-top fluctuation ≤ 1% for stable ion extraction. Fourth, high-speed gate drive optimization meets the stringent driving requirements of GaN switches. Isolated high-speed dual-channel gate drivers with peak output current ≥ 10 A, propagation delay ≤ 5 ns and channel mismatch ≤ 500 ps enable rapid charging/discharging of GaN gate capacitance for nanosecond switching. Strong forward triggering accelerates turn-on, while negative turn-off bias prevents false triggering. Drivers are placed extremely close to power devices with drive loop length ≤ 3 mm to minimize parasitic inductance and ringing. Fully consistent drive circuit design across all stages ensures uniform delay and further improves multi-stage synchronization. Fifth, high-stability and high-reliability design ensures long-term continuous operation for TOF-MS. Amplitude stability is maintained via closed-loop charging feedback and real-time pulse calibration using high-speed ADCs at 10 kHz update rate, achieving long-term amplitude stability ≤ ±0.2%/8 h. Temperature compensation dynamically corrects charging voltage and drive parameters against thermal drift, limiting amplitude variation ≤ ±0.3% across 0 ℃~50 ℃. Timing stability relies on OCXO temperature control and online calibration using high-resolution TDCs to continuously measure and correct multi-channel delay, maintaining synchronization accuracy ≤ 1 ns throughout the equipment lifespan. Comprehensive dual hardware/software protection includes input under/overvoltage, output overvoltage, overcurrent/short-circuit, overtemperature and ESD protection with hardware response ≤ 100 ns to instantly disable pulses and protect the expensive ion optical system. Safety interlocks coordinate with the mass spectrometer to halt ion source and laser operation during faults. Multi-channel coordinated control and spectrometer integration serve as critical supports for system-level operation. Flexible multi-channel timing linkage synchronizes ion repulsion, ion gate, reflector, deflector, laser trigger and detector acquisition with 100 ps resolution. Predefined timing templates for proteomics, metabolomics, small-molecule and macromolecule analysis allow one-click switching to improve usability. Full parameter programmability via Ethernet, RS485 or USB enables remote configuration of amplitude, width, delay and repetition frequency, alongside real-time waveform monitoring, fault alarms and data logging for automated analysis. Standard trigger interfaces achieve seamless synchronization with lasers, analyzers and data acquisition modules. Fully shielded mechanical design complies with GB/T 17626 Class B emission standards to avoid interference with weak ion signals; optimized star-point grounding eliminates ground-offset noise in detection circuits. All core components adopt high-reliability industrial-grade selection with Class I derating (voltage ≤70%, current ≤60%, temperature ≤80% of ratings). Environmental cycling, aging and vibration tests verify an MTBF ≥ 50,000 hours to support uninterrupted laboratory operation. In summary, this methodology forms a complete technical framework covering nanosecond pulse topology, sub-nanosecond synchronization, waveform optimization and long-term stability, resolving the core drawbacks of traditional high-voltage pulse power supplies regarding poor synchronization, large jitter and slow pulse edges. The all-solid-state Marx topology with GaN devices realizes pulse edges within 10 ns; all-fiber triggering plus FPGA hardware control delivers multi-channel synchronization within 1 ns and jitter below 500 ps; full temperature compensation ensures outstanding long-term stability. Fully compliant with the nanosecond-level synchronization demands of high-end TOF-MS, it is widely applicable to various time-of-flight mass spectrometers, providing core technical support for domestic independent development and performance upgrading of high-end Chinese mass spectrometry instruments.