The chemiluminescence immunoassay analyzer is a core device in the in‑vitro diagnostic (IVD) field. Featuring high sensitivity, wide detection range, rapid testing and high automation, it is widely used in clinical scenarios such as tumor marker detection, infectious disease diagnosis, endocrine hormone assays, cardiovascular marker analysis and thyroid function testing, serving as essential standard equipment in hospital clinical laboratories and third‑party medical testing institutions. The multi‑channel high‑voltage bias power supply is a critical supporting component of chemiluminescence immunoassay analyzers, providing independently adjustable, high‑precision high‑voltage bias for multiple photomultiplier tubes (PMTs). Its inter‑channel crosstalk suppression capability, output voltage accuracy, thermal stability and low‑noise performance directly determine the gain stability, detection sensitivity, limit of detection and result repeatability of the PMT system. Current fully automated chemiluminescence immunoassay analyzers are typically equipped with 8~16 independent detection channels, while high‑throughput models adopt more than 32 channels. Typical technical specifications require continuous adjustable single‑channel output of 0~1500 V with voltage regulation accuracy better than ±0.1%, inter‑channel crosstalk below 0.05%, output voltage temperature coefficient less than 5 ppm/°C, and peak‑to‑peak output ripple lower than 10 mV. Failure to meet these criteria causes inconsistent PMT gain across channels, excessive coefficient of variation (CV) in test results, and inability to accurately detect trace markers at the pg/mL level. Traditional multi‑channel high‑voltage power supplies adopt a single‑inverter multi‑output topology, suffering from severe inter‑channel crosstalk, poor output consistency and lack of independent channel tuning, making them incompatible with the high‑sensitivity and high‑throughput requirements of modern automated chemiluminescence analyzers. All relevant designs must comply strictly with IEC 61010‑1 safety requirements for electrical equipment in measurement, control and laboratory applications, and GB 4793.1 safety standards for measuring, controlling and laboratory electrical equipment, while meeting industry specifications and registration requirements for IVD devices. Addressing the core application demands and technical challenges of multi‑channel high‑voltage bias power supplies for chemiluminescence immunoassay analyzers, this methodology establishes a comprehensive technical framework covering multi‑channel topology design, inter‑channel crosstalk suppression, low‑noise output optimization, high‑precision stability control and safety protection. It satisfies multi‑channel high‑voltage power demands for various IVD devices including chemiluminescence analyzers, biochemical analyzers and molecular diagnostic instruments, providing standardized design guidelines to support domestic substitution and performance upgrading of local in‑vitro diagnostic equipment. Focusing on independent multi‑channel control, ultra‑low crosstalk and low‑noise requirements in chemiluminescence systems, this methodology adopts a main architecture of “single‑channel independent modular inversion + distributed digital control”. Each detection channel corresponds to a fully independent high‑voltage power module sharing only the primary input power and system controller. Every module integrates dedicated inversion, voltage boosting, rectification, filtering, closed‑loop regulation and protection circuits, enabling independent start‑stop, voltage tuning and fault protection per channel. This fundamentally eliminates the electrical coupling and crosstalk inherent in conventional shared inverter topologies. Five core design principles are implemented. First, each single‑channel module adopts a flyback inverter topology, offering simple structure, compact size, high input‑output isolation and high voltage gain ideal for modular multi‑channel miniaturization. Quasi‑resonant soft switching minimizes switching loss and electromagnetic interference while easily covering the full 0~1500 V output range required for PMT biasing. Optimized high‑frequency ferrite transformers use sandwich winding to enhance coupling and reduce leakage inductance with reinforced insulation to satisfy isolation specifications. Second, all power modules maintain fully standardized and identical design in circuit topology, component selection, PCB layout and mechanical dimensions to ensure complete interchangeability. The system can flexibly configure 8‑channel, 16‑channel, 32‑channel or higher throughput according to instrument demands, while supporting independent maintenance and replacement without affecting other channels. Third, strict physical and electrical isolation is enforced between channels. Each module is enclosed in an independent metal shielding compartment separated by solid metal partitions to suppress radiative crosstalk. Every input integrates dedicated EMI filtering and isolation circuits to block conducted interference through power lines. Each output incorporates independent high‑voltage filtering and isolation diodes to eliminate coupling crosstalk, while control signals adopt isolated power supplies and optocoupler interfaces to fully separate power and signal domains. Fourth, a distributed two‑level digital control architecture is implemented with a main system controller (ARM/FPGA) managing overall communication, command distribution and status monitoring, while each embedded slave MCU performs local closed‑loop regulation and protection. Isolated I²C/SPI communication prevents control‑induced crosstalk and ensures synchronous independent channel operation. Fifth, low‑power miniaturization limits each module rated power below 5 W to match the microampere load of PMTs. High‑density surface‑mount packaging restricts single module volume within 3 cm×2 cm×1 cm, enabling dense integration inside confined analyzer housings. Inter‑channel crosstalk suppression represents the core of this methodology. Comprehensive crosstalk reduction strategies cover architectural isolation, conducted interference suppression, radiative shielding and grounding optimization. Architectural independence eliminates shared power paths, common transformers and combined switching circuits, achieving measured crosstalk below 0.03%, exceeding the industry standard of 0.05%. Conducted interference is suppressed through dual‑stage π‑mode EMI filtering per channel, isolated power chokes, decoupling diodes and fully isolated communication buses with independent termination and ESD protection. High‑voltage output lines employ shielded cables with single‑ended grounding to eliminate capacitive coupling between channels. Radiative crosstalk is minimized using permeable alloy or aluminum alloy shielding enclosures with individual compartment grounding. PCB layouts strictly separate high‑voltage/low‑voltage domains and power/signal paths while adopting staggered switching frequencies to avoid synchronous interference. The entire power system is enclosed in a sealed metal chassis for secondary shielding. Grounding architecture adopts star‑type single‑point grounding with separated power ground, signal ground and shield ground to eliminate ground loops and potential difference crosstalk. High‑voltage and low‑voltage grounds remain fully isolated, while power and signal grounds connect only through dedicated magnetic beads to block noise coupling into precision control circuits. Low‑noise high‑stability output optimization ensures ultra‑clean biasing for trace detection. Multi‑stage high‑voltage RC filtering combined with quasi‑resonant soft switching suppresses peak‑to‑peak ripple below 5 mV, far better than the typical 10 mV requirement, preserving weak luminescence signals and improving signal‑to‑noise ratio. Low‑leakage silicon stacks or SiC rectifier diodes eliminate reverse recovery noise, while low‑ESR polystyrene or polypropylene film capacitors prevent piezoelectric noise artifacts. Precision temperature compensation adopts ultra‑low drift bandgap references (≤2 ppm/°C) and high‑precision metal‑film resistors (≤5 ppm/°C). Integrated temperature sensors enable real‑time drift modeling and dynamic PWM correction, ensuring overall temperature coefficient ≤3 ppm/°C across 0°C~50°C and long‑term 8‑hour drift below 0.1%. High‑resolution digital control using 16‑bit DAC and 24‑bit ADC achieves fine 0.1 V tuning steps with resolution better than 0.01%. Advanced digital PID closed‑loop regulation guarantees voltage accuracy within ±0.05%, load regulation within ±0.1% and line regulation within ±0.05%. Full‑range multi‑point calibration ensures consistent output matching across all channels within ±0.1%, stabilizing PMT gain uniformity and improving batch‑to‑batch assay repeatability. Safety protection and reliability comply fully with IEC 61010‑1 and GB 4793.1 laboratory equipment standards with reinforced double insulation and ≥3 kVAC isolation. All high‑voltage sections are fully enclosed with limited leakage current below 0.5 mA. Each channel integrates independent hardware‑software dual protection including over/under input voltage, output overvoltage, overcurrent/short circuit, overtemperature and open‑circuit detection with fault response faster than 1 μs. Any single‑channel failure triggers localized protection without affecting other working channels while reporting diagnostic data to the main controller. All critical components follow strict medical‑grade derating with voltage stress ≤70%, current stress ≤60% and temperature stress ≤80% of rated values. Full validation includes temperature cycling, long‑term aging and vibration testing, achieving an MTBF exceeding 30,000 hours suitable for continuous laboratory operation. Complete EMC compliance with the GB/T 17626 series ensures stable performance in complex clinical electromagnetic environments. In summary, this methodology delivers a full‑solution framework covering multi‑channel topology innovation, rigorous crosstalk elimination, ultra‑low‑noise optimization and comprehensive safety design. It fundamentally resolves the traditional limitations of severe inter‑channel interference, inconsistent output and poor independent adjustability in conventional high‑voltage power supplies. Modular independent channel design achieves crosstalk suppression below 0.03%, advanced multi‑stage filtering reduces output noise to under 5 mV, and full temperature compensation stabilizes drift within 3 ppm/°C. Fully adapted to high‑sensitivity high‑throughput trace detection requirements of modern chemiluminescence immunoassay analyzers, this technology provides core technical support for the upgrading and domestic localization of high‑performance IVD instruments.